Standard Rf Values - Biology (Photosynthesis) - Physics Forums
Mar 23, 2006 · Research the effects of different solvents on Rf values in chromatography Learn about the extraction methods for plant pigments Explore advanced chromatography …
Effect of Solvent Polarity on Rf Value in TLC - Physics Forums
Aug 3, 2013 · The polarity of the solvent significantly influences the Rf value in Thin Layer Chromatography (TLC). Increasing the polarity of the mobile phase enhances the solubility of …
How does the polarity of the eluent and sample affect the Rf value …
Feb 26, 2017 · A three-component mixture (X, Y, Z) was separated using normal phase TLC. Three eluents of varying polarity were used. Select the TLC plate that was developed using …
How can you predict relative Rf values of cis-trans isomers in thin ...
The cis,trans-isomer cannot adopt a planar conformation because of steric hindrance between the two phenyl rings. The non-coplanar geometry lowers the efficiency of interactions with the …
What Are the Standard Rf Values for Plant Pigments in Spinach ...
Oct 25, 2004 · The standard Rf values for plant pigments in spinach chromatography can vary significantly based on the solvent used, with a common solvent being a mixture of 90% …
If the polarity of the solvent increases, will the Rf value increase or ...
True or False: A sample of aspirin has an Rf R f value of 0.20 when measured on a silica gel TLC plate with a certain solvent mixture. If the polarity of the solvent is increased, the Rf R f value …
analytical chemistry - How does the Rf value change when …
May 29, 2016 · A non-polar molecule X is analyzed by TLC chromatography on silica to determine its Rf R f value. The first eluent was hexane:EtOAc = 9: 1 9: 1 and the Rf R f value was 0.5 …
organic chemistry - Reasoning retention factor of benzoic acid ...
Oct 18, 2016 · The answer is the dimer formation of the benzoic acid. This is why higher concentrations lead to more extended dimer formation, thus higher Rf R f values as the …
Help Identifying Pigment by Rf Values - Physics Forums
Oct 26, 2007 · The discussion focuses on identifying pigments based on Rf values obtained in a lab setting. The user has established that an Rf value of 0.23 corresponds to Neoxanthin and …
RF Measurement: Do I Need a Unit? - Physics Forums
Nov 24, 2004 · Rf, or retention factor, is a ratio used in chromatography that does not have units. When measuring distances in centimeters (cm) for thin-layer chromatography (TLC), the units …