The Applied Biosystems™ QuantStudio™ Real-Time PCR (qPCR) instruments build on innovations developed by Thermo Fisher Scientific™ chemists and engineers. The Applied Biosystems™ VeriFlex™ system, ...

Object Details Author Pelt-Verkuil, Elizabeth van 1951- Belkum, Alex van Hays, John P Contents The polymerase chain reaction -- A brief comparison between in vivo DNA replication and in vitro PCR ...

Next-Generation PCR Instruments Boost Workflows Modern, intuitive, and reliable thermal cyclers excel at optimizing sequencing, cloning, and genotyping throughput.

A variety of PCR protocols have been proposed to minimize these problems, most of which rely on high template concentrations and low numbers of PCR cycles 2, 3.

PCR-based methods offer advantages over more traditional diagnostic tests, in that organisms do not need to be cultured prior to their detection and protocols are highly sensitive and rapid.

In conclusion, the M13 (-21) primer genotyping protocol offers an inexpensive alternative to the use of commercially available fluorescent labeled dye primers—an advantage especially for small ...