GEN

Assessing qRT-PCR COVID-19 Testing Results: What Can Go Wrong?

COVID-19 is commonly diagnosed by quantitative fluorescence real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) (1, 2). The test is the sequential action of two enzymes–an ...
Nature

RNA amplification and cDNA synthesis for qRT-PCR directly from a single cell

Although qRT-PCR studies provide valuable information about the relative expression of transcripts in a cell, they are difficult to perform with very small numbers of cells (for example, 1–1,000 cells ...
GEN

Technical Note: Highly Sensitive qRT-PCR from One Cell

Ideally, gene expression studies by quantitative RT-PCR (qRT-PCR) should be performed using RNA from homogeneous cell populations. Frequently, this means performing qRT-PCR using picogram amounts of ...
Nature

3D microdevices that perform sample purification and multiplex qRT-PCR for early cancer detection with confirmation of specific RNAs

MicroRNA expression analysis is an important screening tool for the early detection of cancer. In this study, we developed two portable three-dimensional microdevices for multiple singleplex RNA ...
Nasdaq

Agilent Completes CE-IVD Registration of qRT-PCR In Vitro Diagnostic Kit for SARS-CoV-2 RNA Detection

SANTA CLARA, Calif.--(BUSINESS WIRE)-- Agilent Technologies Inc. (NYSE: A) today announced the launch of a real-time reverse transcription (qRT) PCR-based diagnostic kit for the detection of ...
technologynetworks

RT2 Profiler™ PCR Arrays: Pathway-Focused Gene Expression Profiling with qRT-PCR

This paper evaluates the performance of the newest technique for monitoring the expression of a panel of pathway- or disease-specific genes: the RT 2 Profiler PCR Array System from SA Biosciences. The ...