Human umbilical vein endothelial cells (HUVECs) were seeded at 1 × 10 4 cells per cm 2 in six-well plates. Treatment with TNF-α (2.5, 5 or 10 ng/ml) or lipopolysaccharide (0.5, 1 or 5 μg/ml) treatment ...

An efficient, robust method of examining the interactions between cells uses fluorescent tagging to simultaneously analyze multiple cell populations, speeding up a once tedious and limiting process.

Researchers from the University of Freiburg, Germany have developed a functional flow cytometry-based method for the diagnosis of innate primary immunodeficiencies (PIDs). The technique was applied to ...